Ihe nzacha nnyonye anya fluorescence
Nchịkọta ngwaahịa
Fluorescence filter bụ akụkụ dị mkpa ejiri na ngwa sayensị biomedical na ndụ.Isi ọrụ ya bụ ikewapụ wee họrọ ụdịdị dị iche iche njiri mara ogologo ikuku sitere na ọkụ mkpali na ikuku ikuku nke ihe dị na nyocha na usoro nyocha nke biomedical fluorescence.A na-eji ihe nzacha fluorescence mara ya site na omimi mbelata dị omimi yana obere autofluorescence.Ọtụtụ mgbe, ọtụtụ ihe nzacha nwere ike jikọta ọnụ iji mepụta nzacha fluorescence, nke nwere arụmọrụ dị elu.
Ubi ngwa
A na-eji ihe nzacha fluorescence eme ihe na ngwa PCR nke fluorescence n'ezie.nke a na-ejikarị na usoro ihe ọmụmụ ihe omimi na nchọpụta nchekwa nri na nlekota ahụike ọha na eze na ụlọ ọrụ ndị ọzọ.
A na-eji nzacha fluorescence maka ịchọpụta ihe dị iche iche e ji esiji ákwà na nyocha, dịka ọmụmaatụ:
FAM / SYBR Green / Green / HEX / TET / Cy3 / JOE / ROX / Cy3.5 / Texas Red, Cy5 / LC Red640, Cy5.5 wdg
| Usoro | (IAD Hard mkpuchi) | ||
| Ogologo ogologo | Ex (nm) | Em(nm) | Gafee |
|
| 470-30 | 525-20 | > 6 |
|
| 523-20 | 564-20 | > 6 |
|
| 543-20 | 584-20 | > 6 |
|
| 571-20 | 612-20 | > 6 |
|
| 628-35 | 692-45 | > 6 |
| Mgbochi | OD> 6@200 ~ 900nm ma ọ bụ @200 ~ 1200nm | ||
| Mkpọda(nm) | 50% ~ OD5 <10nm | ||
| Gafee | OD>6 | ||
| Nha (mm) | Φ4mm, Φ12mm, Φ12.7mm, Φ25.4mm wdg | ||
Spectrum
Usoro mmepụta
